Here, FR+ and FR-negative tumor cells without or with EC17 pre-loading (0.1 or 10 nM, 30 min pulse at 37°C) were incubated with EGFRt-sorted CAR-T cells (1:1 CD4/CD8) at an E/T ratio of 1:2 (Figures 4B,D). Here, CD4 is linked to neoplasm.