Our study shows that high motility gliomas expressing Cx43 differ from their parental counterparts by the upregulation of MMP3, osteopontin, aldo-keto reductase family 1 member B10 (ARK1B10), and to a lesser degree SERPINE2 (2.5-fold, P = 0.03), and phosphoglycerate kinase 1 (2.4-fold, P = 0.04). Here, PGK1 is linked to central nervous system cancer.