As outlined in Table 1, the top 6 functional annotations and disease processes differentiated by the Cx43 secretome (by rank) Metastasis (P = 5.51 × 10-7, Activation Z-Score +0.132), Aggregation of Cancer Cells (P = 3.36 × 10-6), Disruption of Cytoskeleton (P = 4.00 × 10-6), Metastasis of Tumor cell lines (P = 4.99 × 10-6, Activation Z-Score = +0.132), Breast/Colorectal Cancer (P = 5.57–5.97 × 10-6) and Advanced Malignant Tumor (P = 9.13 × 10-6, Activation Z-Score = +0.297). The gene discussed is GJA1; the disease is colorectal cancer.