To provide independent support for a role for tumor-specific T cells in the antitumor response of Rnf5−/− mice, we transferred OVA-specific OT-I transgenic CD8+ T cells into WT or Rnf5−/− recipient mice and then injected recipients subcutaneously with OVA-expressing B16F10 melanoma cells. Here, CD8A is linked to neoplasm.