This panel of TKIs indeed induced signs of ICD such as immunofluorescence-detectable CALR exposure and the release of ATP and HMGB1 into the culture supernatant in several human cancer cell lines (U2OS, cervical carcinoma HeLa, colorectal cancer HCT-116) and in murine fibrosarcoma MCA205 cells (Supplementary Fig. 1a–c). Here, CALR is linked to colorectal cancer.