Via DNA extraction and bisulfite sequencing, respectively, we found uniform hypermethylation at CpG and non-CpG sites within the promoter and first exon of the HIF-1α gene in luminal subtype breast cancer samples (Fig. 3a upper panel) and hypomethylation in TNBC samples (Fig. 3a bottom panel). The gene discussed is HIF1A; the disease is breast cancer.