In order to evaluate the effect of miR‐204 on PCAT6 expression, these two CRC cell lines were transfected with miR‐204 mimics or miR‐204 inhibitor, as confirmed by real‐time PCR assays (Figure 3B); as revealed by real‐time PCR assays, PCAT6 expression was negatively regulated by miR‐204 in CRC cells (Figure 3C). The gene discussed is PCAT6; the disease is colorectal carcinoma.