In contrast, the expression of ACTA1 showed an obviously downward trend between well and moderately or poorly HNSCC samples; the mean LS of ACTA1 in well-differentiated HNSCC was 64.78 ± 9.400%; however, the results of LS between moderately and poorly differentiated HNSCC were all zero. The gene discussed is ACTA1; the disease is head and neck squamous cell carcinoma.