The role of Gab2/3 in macrophages during colitis development was next assessed, where 1 × 106 LPS-polarized BMDMs from Gab2/3−/− or WT were adoptively transferred into Rag2−/− mice first, and then sorted naïve WT CD4+ T-cells were transferred 24 h later. This evidence concerns the gene RAG2 and colitis.