Furthermore, fatty acids are usually used to induce TG accumulation in culture hepatocytes,(29,30) but the fatty acids-induced fatty liver model is unsuitable for the evaluation of the molecular crosstalk between FXR and PPARα because fatty acids are natural PPARα ligand.(26,27) This cell culture model of fatty liver induced by LXRα-ligands should be useful for evaluating the different mechanism of lipid metabolism for each species. The gene discussed is PPARA; the disease is fatty liver disease.