MAP1LC3B and hepatocellular carcinoma: To examine whether WFA induces autophagosomes in HCC cells, we transfected Huh7 and MHCC97H cells with mRFP-EGFP-LC3B, a tandem fluorescent-tagged LC3B plasmid that can identify autophagosomes (GFP-positive and RFP-positive merged as yellow) and autophagolysosomes (GFP-negative and RFP-positive merged as red) [36].