Formation of autophagolysosomes was observed as a merge of autophagosomes (marked with GFP-LC3B, green signal) and lysosomes (marked with RFP-Rab7, red signal) and displayed as yellow signals (GFP positive/RFP positive) in HCC cells treated with WFA similar to EBSS-treated cells, while untreated cells showed weak red and green signals (Figure 4B). The gene discussed is MAP1LC3B; the disease is hepatocellular carcinoma.