To further explore the underlying molecular mechanism of the antiviral effects of the eIF4F complex on rotavirus infection, we questioned the effect of the eIF4F complex on several antiviral proteins including interferon regulatory factor 1 (IRF1), IRF7, and retinoic acid-inducible-I (RIG-I), which indicated that the knockdown of eIF4A had no obvious effect on these antiviral proteins. Here, IRF1 is linked to Rotavirus infection.