In addition to the phosphorylation of Ser727 and/or Tyr705 of STAT3, nuclear translocation of activated STAT3 is required for transcriptional activation.35, 36, 37 Thus, translocation of activated p‐STAT3 into the nucleus was examined by the subcellular fractionation and immunofluorescence staining of colon cancer cells (Figure 3B,C). This evidence concerns the gene STAT3 and colonic neoplasm.