Furthermore, these undifferentiated germ cells had proliferation and apoptosis ability (TUNEL and PH3 positive; Fig. S2b and S2c) without normal niche environments (Zfh1 positivity with abnormal pattern, no Eya-positive cyst cells, and no FasIII-positive hub cells; Fig. 1b, c). This evidence concerns the gene PHC3 and cyst.