Given the immunogenic nature of melanoma (18), as well as the poor prognosis associated with metastatic disease, we sought to objectively determine the immune cell infiltration (Immunoscore) and PD-L1 status of both primary tumors and metastases in a retrospective cohort based study of patients with metastatic melanoma, treated with anti-CTLA-4 and/or anti-PD-1 antibodies. The gene discussed is CTLA4; the disease is melanoma.