Inhibition of CXCR4 activity by a single s.c. injection of AMD3100 (100 μg/foot skin), a selective CXCR4 antagonist, administered 2 h prior to infection failed to alter viral loads in the pLN of Wt and TLR7−/− mice as compared to vehicle treated littermate controls (Supplementary Figure 4C). Here, TLR7 is linked to infection.