We used multicolor flow cytometric analysis to determine SIGLEC1 expression at the protein level in an independent cohort of breast cancer patients and found that SIGLEC1 was expressed on Br-TAM, but not on other immune cells or CD45− non-immune cells, indicating specificity to macrophages/TAMs (Figures S3A and S3B). This evidence concerns the gene SIGLEC1 and breast carcinoma.