Analysis of the changes in miRNA and mRNA expression between the gemcitabine-resistant pancreatic cancer cell line PANC-1 and the parental pancreatic cancer cell line based on GSE80616 and GSE80617 datasets in the GEO database revealed that both miR-30a-5p and CD73 had the most significant changes. The gene discussed is NT5E; the disease is familial pancreatic carcinoma.