Further testing the activity of GSK-3β in the GAS-infected RAW264.7 cells revealed that a reduction of GSK-3β serine 9 phosphorylation, which was an indication of GSK-3β inactivation, was observed both in the wild type GAS- and the sagB mutant-infected RAW264.7 cells at 3 h post-infection. Here, GSK3B is linked to infection.