By flow cytometry we found that a greater number of intra-renal Ly6C− macrophages (CD45+CD11b+F4/80+Ly6C−) occurred in the kidneys from CCR2−/− mice compared to WT (Fig. 2e, f), while Ly6C+ macrophages showed no significant difference in the CKD phase compared to WT mice (Fig. 2g). This evidence concerns the gene CCR2 and chronic kidney disease.