To investigate whether miR-96-5p may promote cell migration and cell resistance to therapy by silencing the PTEN expression in HNSCC cells, we assessed the effect of PTEN depletion on cell migration, clonogenicity and sensitivity to radiotherapy by the transfection of PTEN siRNA for 48 h in Cal 27 cell line. The gene discussed is PTEN; the disease is head and neck squamous cell carcinoma.