In present work, we for the first time verified that PGK1 directly binds HSP90 and that overexpression of PGK1 down-regulates cellular ATP level in endometrial cancer cell lines, which is consistent with previous finding that HSP90 ATPase is activated by terazosin through PGK1 under cellular stress (Chen et al., 2015). This evidence concerns the gene PGK1 and endometrial cancer.