BECN1 and fatty liver disease: To investigate the molecular mechanisms responsible for the reduction of hepatic steatosis upon trehalose treatment in the liver of apoE−/− mice on an HFD, we performed immunoblotting and real-time PCR of key factors related to autophagy: Beclin-1, microtubule-associated proteins 1A/1B light-chain 3B (LC3), and autophagy-related protein 7 (APG7).