LncRNA ENST00000413528 is overexpressed in both human glioma tissues and U251 and LN229 cells; additionally, the knockdown of ENST00000413528 in glioma cells inhibits cell proliferation and colony formation abilities and induces the G0/G1 arrest of the cell cycle, and promotes apoptosis via a miR‐593‐5p/PLK1 pathway. Here, PLK1 is linked to central nervous system cancer.