Our experimental results also demonstrated that the effects of the downregulation of ENST00000413528 and upregulation of miR‐593‐5p on glioma cells were consistent; there was a decrease in the expression of the PLK1 protein, inhibition of cell proliferation and colony formation, inducement of the G0/G1 arrest, and promotion of the apoptosis of glioma cells. This evidence concerns the gene PLK1 and glioma.