Fos staining in the LH of Gi DREADD + CNO + 3% HTN rats had significantly less Fos expression than the CTRL + CNO + 3% HTN-treated rats (p = 0.001, Tukey’s post hoc analysis; Fig. 9B) and significantly higher than both the 0.9% SAL-treated groups (both p < 0.001, Tukey’s post hoc analysis; Fig. 9B). This evidence concerns the gene FOS and hypertensive disorder.