To assess the methylation pattern of PCDH17 in primary AML samples and to see whether its repression is mediated by methylation, we used targeted bisulfite sequencing to measure the methylation levels of 20 CpG sites in the first exon of PCDH17 (chr13:58,206,837-58207013). Here, PCDH17 is linked to acute myeloid leukemia.