Immunohistochemical results showed substantial Nephrin and Synaptopodin immunoreactivity in the cytoplasm of the Ctr, Sham, and AKI 20 groups, with only small amount of immunoreactivity in the podocyte cytoplasm of the AKI 30 and AKI 40 groups (Fig. 3). Here, NPHS1 is linked to acute kidney injury.