The glycolysis rate strongly depends on the upregulated expression and activity of glucose transporters (Gluts), such as Glut1, which is regulated by HIF‐1α and is considered the main overexpressed Glut, with a 10‐12‐fold higher expression in tumours than in normal cells.49, 51 In this study, the expression levels of Glut 1, LDH‐A and HK2 were detected to assess the glycolysis rate and the results indicated that PD and 2‐DG co‐treatment inhibited glycolysis in breast cancer cell lines. The gene discussed is HK2; the disease is breast cancer.