However, previous studies have shown that 2‐DG induces the phosphorylation of AKT and the efficacy of clinical trials has previously been limited by the systemic toxicity.41, 42 The activation of AKT is commonly observed in a variety of cancers and seems to be intricately associated with aerobic glycolysis, proliferation and invasiveness.43, 44 In this study, we found that the mechanism of action of the treatment combination is that PD blocks the activation of the PI3K/AKT pathway by 2‐DG (Figure 3). This evidence concerns the gene AKT1 and cancer.