To evaluate whether increased nuclear β-catenin is transcriptionally active, we used paired TOP-Flash (which is subjected to T-cell factor/β-catenin transactivation) and FOP-Flash control luciferase reporters and found that β-catenin transactivation activity was increased upon FBXW2 knockdown, but reduced upon FBXW2 overexpression in multiple lines of lung cancer cells (Fig. 2e). This evidence concerns the gene FBXW2 and lung cancer.