Immunofluorescence images of spermatocyte cysts from flies that carry a transgene expressing Drosophila E-cadherin [encoded by shotgun (shg)] tagged with the fluorescent protein Tomato (Shg::Tomato), showed that the cyst cell-cyst cell junction has typical apicobasal polarity domains, in which the Par complex and adherens junction proteins colocalize (Fig. S2C), whereas the basolateral domain protein Dlg was juxtaposed to but did not colocalize with Shg and Baz (Fig. S2D). Here, PARD3 is linked to cyst.