ASB2 and neoplasm: Following validation of ASB2 methylation and expression data from sequencing via MBD-qPCR and RT-qPCR, respectively, in both KO and parental cells (Additional file 17: Figure S11 and Additional file 18: Figure S12), we examined methylation levels of our TET2-KO-specific DMR in an independent, limited series of primary patient samples comprised of matched normal and tumor tissues (n = 19 per group).