In this paper, we showed that TNF-β, similar to TNF-α, stimulated distinctive morphological and molecular changes from epithelial to fibroblast-like mesenchymal phenotypes (EMT) with the formation of filopodia and lamellipodia, and this was accompanied by EMT factors (increased vimentin and EMT-specific transcription factor slug, decreased E-cadherin) in all three CRC cell lines. Here, TNF is linked to colorectal carcinoma.