In the present report, we demonstrated by immunoprecipitation assay that the impact of TNF-β-promoted CRC cell invasion and EMT points to a functional connection between TNF-βR and tumor-inducing biomarkers, such as FAK and NF-κB, suggesting that TNF-β increased the expression of TNF-βR, leading to FAK and NF-κB activation. This evidence concerns the gene TNFRSF1B and colorectal carcinoma.