Identification the effective concentration of ferutinin on bone mineralization of zebrafish larvae at 2 dpf could be helpful for the future study to find the effect of ferutinin on healing the osteoporosis using prednisolone and dexamethasone to induce osteoporosis in transgenic zebrafish such as tg (sp7:egfp) to monitor bone formation directly. The gene discussed is TG; the disease is osteoporosis.