In general, the resultant populations of SD NCSCs and HF NCSCs obtained after the large-scale expansion had the same immunophenotype, SOX10+CD271+Nestin+CD73+CD90+CD105+CD140a+CD140b+CD146+CD166+CD349+CD34− CD45− CD56− CD117−HLA-DR−, showed a similar level of gene expression, produced the same spectrum of chemokines, cytokines, and growth factors, and also had the ability to undergo directed multilineage differentiation and formation of neurosphere-like structures. The gene discussed is ENG; the disease is hydrops fetalis.