In the present study, we show that PMCA-treated urine PrPD is competent to transmit to Tg mice expressing human PrP a prion disease that, as to the histopathological phenotype and resPrPD properties, is indistinguishable from the disease transmitted by inoculation with vCJD BH. Here, PRNP is linked to variant Creutzfeldt-Jakob disease.