THP-1 and DN-IκBα THP-1 cells were infected or not with HSV-1 at MOI 50 PFU/cell and collected at 3, 18, 24 and 48 hrs p.i. The significant induction of miR-146a upon HSV-1 infection occurred in wild type THP-1 cells (wt THP-1) only and not in DN-IκBα THP-1 cells; as shown by qPCR analysis its expression reached a peak at 48h post infection indicating a specific response related to HSV-1 replication (Fig. 5a). Here, NFKBIA is linked to infection.