Furthermore, to investigate the ability of tumor antigen experienced HPK1 KD T cells to elicit an anti-tumor response, splenocytes from WT and HPK1 KD tumor bearing mice were cultured with irradiated 1956 tumor at different effector and target cell ratios for 4hr followed by the measurement of tumor cell lysis using LDH release. This evidence concerns the gene MAP4K1 and neoplasm.