It showed that a 20% tension induced a decreased cell proliferation potency and telomerase activity, an increased G1/G0 phase fraction, and a decreased S phase fraction, as well as an up-regulated gene/protein expression of senescence markers (p16 and p53) compared with the 5% tension group, directly indicating that 20% tension significantly promotes AF cell senescence compared with 5% tension. The gene discussed is TP53; the disease is atrial fibrillation.