WRN and endometrial carcinoma: In RKO cells expressing WRNr variants, we observed a slightly stronger dependency on WRN helicase ATP-binding function compared to exonuclease activity upon knock-down of endogenous WRN (Figure 4—figure supplement 1A and B), while WRNr-expressing monoclonal lines generated from the MSI endometrial carcinoma model HEC-265 showed an exclusive dependency on WRN helicase function (Figure 4—figure supplement 1C), similar to HCT 116 cells.