Because high expression of SALL4 has been reported in breast cancer cell MCF7 and lung cancer cell A549, these two cell lines were employed as positive controls.31, 32 Results showed that SALL4 expression of CNE2 cells was at a moderate level, whereas that of CNE2R cells was higher at both protein and RNA levels (Figure 2C, D). The gene discussed is SALL4; the disease is breast carcinoma.