Here, in this study, to investigate the differential expressed proteins (DEPs) of aberrant MAP4 phosphorylation in vivo and dig out the underlying mechanism that mediating MAP4 phosphorylation-induced mitochondrial dysfunction which is responsible for cardiomyopathy, we generated mice that mimicked MAP4 hyperphosphorylation at specific sites (S737 and S760). The gene discussed is MAP4; the disease is cardiomyopathy.