CHAT and myeloid sarcoma: Therefore, in light of earlier studies in rat MS/DB, reporting putative synaptic contacts between NI projections (and relaxin-3-positive elements) with both PV- and ChAT-positive neurons in MS/DB (Olucha-Bordonau et al., 2012), a triple-label immunohistochemistry protocol was employed in an effort to analyze putative contacts between relaxin-3 nerve fibers/terminals and PV- and/or ChAT-positive neuronal soma and processes in mouse MS/DB.