The tumor‐associated proinflammatory cytokines and gelatinases have been identified as the key factors in enhancing GBC tumorigenesis and progression.13 Among these elements, IL‐6, IL‐1β, TNF‐α and MMP9 are the pivotal cytokines and gelatinase presented in the tumor microenvironment.26, 27 Therefore, we analysed the effects of SP/NK‐1R system on these tumor‐associated mediators in GBC‐SD and NOZ cells. This evidence concerns the gene IL1B and neoplasm.