Using HEK293T cells as a positive control (Fig. 7a), we found that DRAM1 inhibited rpS6 phosphorylation in human colon cancer cells, SW480 (Fig. 7b) and HCT116 (Fig. 7c), with phosphorylation at Ser235/236 more significantly affected by DRAM1 than the site at Ser240/244 (Fig. 7d and e). Here, RPS6 is linked to colonic neoplasm.