We carried out an ultra-sensitive RNAscope ISH assay to study the altered NRXN3 expression at cellular levels in control and AD postmortem brain samples that were co-hybridized and co-stained with inflammasome component NLRP3 [41] and neuron marker NEUN/RBFOX3. The gene discussed is NRXN3; the disease is Alzheimer disease.