In contrast, IFN stimulation of carcinoma cells (U2OS, SAOS, HeLa, and A549) failed to induce the recruitment of HIRA to PML-NBs (Fig 3G, S5 Fig; [17]), even though these cell types expressed similar levels of HIRA mRNA and protein within the nucleus (Fig 3H, S5 Fig). This evidence concerns the gene PML and carcinoma.