PML and infection: These data demonstrate that stable recruitment of HIRA to vDNA occurs with temporally distinct kinetics to that of core PML-NB host factors (Fig 1; [9]), and provides supporting evidence that ΔICP0 HSV-1 genomes can be restricted at multiple phases of infection to influence the progression of viral gene expression (Fig 8D–8G, S9D and S9E Fig; [9, 19, 31]).