PML and infection: Consistent with recent studies [9, 10, 64], infecting HSV-1EdC genomes were readily detectible by click chemistry following virion release into the nucleus and to be stably associated with PML-NBs by 90 minutes post-infection (mpi), prior to their disruption and release by ICP0 by 180 mpi (Fig 1A and 1B; [9, 10]).