This was particularly true for nuclear GSK3ß accumulation, which was associated with advanced tumor stage (p < 0.0001), high Gleason grade (p < 0.0001), lymph node metastasis (p < 0.0001), positive surgical margin (p < 0.0001) and high preoperative PSA level (p = 0.0002, Table 1). This evidence concerns the gene KLK3 and metastatic malignant neoplasm in the lymph nodes.