DNA analysis (copy number variations and mutation analysis) of the 12 studied genes (BRAF, NRAS, MAP2K1, MET, CDKN2A, CDK4, CDK6, CCND1, CCND2, CCND3, RB1, CTNNB1) (Supplementary Table 2) was performed in melanoma lesions from 63 patients before treatment (1 missing because of insufficient material). The gene discussed is MAP2K1; the disease is melanoma.