For further selection, we performed statistical analysis between AIS vs. invasive adenocarcinoma and selected 3 CpG sites, ZYG-11 family member A (ZYG11A), LOC10099657, and Mir656 (p < 0.05) as genes that show a significantly lower methylation rate in invasive adenocarcinoma relative to AIS. Here, MIR656 is linked to androgen insensitivity syndrome.