Moreover, by using this method, we identified hypomethylation in 18 other imprinted loci (DIRAS3_Ex2, IGF1R, GNAS-XL, WRB, FAM50B, FANCC, GNAS-A/B, NHP2L1, ERLIN2, MAGEL2, MCTS2P, PPIEL, PEG10, RB1, NDN, SNRPN_5′DMR4, SNRPN_variant4, and L3MBTL1) and hypermethylation in two imprinted loci (ZDBF2 and GNAS-NESP), which were not known to be aberrantly methylated in these five MLID-BWS patients (Table 1). This evidence concerns the gene RB1 and Beckwith-Wiedemann syndrome.