BRAF and colorectal carcinoma: These studies also suggest an exceedingly high level of concordance between primary CRC and metastases in genetic alterations that occur early in colorectal carcinogenesis, such as alterations in APC, KRAS, NRAS, and BRAF. In our study, we achieved an average coverage depth of 124-fold, enabling the sensitive detection of SNVs and indels to a 10% variant frequency.